TTC5 Polyklonaler Antikörper

TTC5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 26112-1-AP

Synonyme

Protein Strap, Strap, Stress-responsive activator of p300

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  WB analysis of Jurkat using 26112-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26112-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 26112-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

26112-1-AP bindet in WB, IHC, ELISA TTC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC5 fusion protein Ag23523
• Vollständiger Name: tetratricopeptide repeat domain 5
• Beobachtetes Molekulargewicht: 45 kDa~49 kDa
• GenBank-Zugangsnummer: BC053538
• Gene symbol: TTC5
• Gene ID (NCBI): 91875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Commun Signal; CARM1 regulates tubulin autoregulation through PI3KC2α R175 methylation; Authors - Yena Cho