TTC7A Polyklonaler Antikörper

TTC7A Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21600-1-AP

Synonyme

TPR repeat protein 7A, KIAA1140

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  WB analysis of K-562 using 21600-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 21600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21600-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse small intestine using 21600-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of PC-3 using 21600-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 21600-1-AP

  IP result of anti-TTC7A (IP:21600-1-AP, 4ug; Detection:21600-1-AP 1:300) with K-562 cells lysate 4000ug.

• 

  IHC staining of mouse small intestine using 21600-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21600-1-AP (TTC7A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SW480-Zellen, K-562-Zellen, Maus-Kolongewebe, Maus-Dünndarmgewebe, Maus-Thymusgewebe, PC-3-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

21600-1-AP bindet in WB, IHC, IF, IP, ELISA TTC7A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC7A fusion protein Ag16212
• Vollständiger Name: tetratricopeptide repeat domain 7A
• Berechnetes Molekulargewicht: 858 aa, 96 kDa
• Beobachtetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: BC114365
• Gene symbol: TTC7A
• Gene ID (NCBI): 57217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTC7A (TPR repeat protein 7A) is expressed in enterocytes within the duodenum, ileum, and colon, and has a role in enterocyte survival and function. Mutations in the TTC7A gene can result in a spectrum of intestinal disease, including multiple intestinal atresia (MIA) and very early onset inflammatory bowel diseases (VEOIBD). Functional analysis revealed that TTC7A binds to and facilitates the transport of PI4KIIIa from the trans-Golgi to the plasma membrane, while loss of TTC7A results in dysregulation of PI4KIIIa signaling. This antibody specially recognizes endogenous TTC7A. (24417819)

Publikationen

Species	Application	Title
human	IHC,IF	Gastroenterology; Mutations in tetratricopeptide repeat domain 7A result in a severe form of very early onset inflammatory bowel disease.; Authors - Yaron Avitzur
human	WB,IHC	Front Immunol; A Novel Homozygous TTC7A Missense Mutation Results in Familial Multiple Intestinal Atresia and Combined Immunodeficiency.; Authors - Wenjun Mou
human	IHC	J Clin Immunol; Bialellic Mutations in Tetratricopeptide Repeat Domain 7A (TTC7A) Cause Common Variable Immunodeficiency-Like Phenotype with Enteropathy.; Authors - Dylan Lawless