BBS8 Polyklonaler Antikörper

BBS8 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12505-1-AP

Synonyme

BBS8, TPR repeat protein 8, TTC8

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12505-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12505-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12505-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12505-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12505-1-AP

  IP Result of anti-BBS8 (IP:12505-1-AP, 3ug; Detection:12505-1-AP 1:400) with HeLa cells lysate 2000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Lungengewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12505-1-AP bindet in WB, IP,ELISA BBS8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS8 fusion protein Ag3186
• Vollständiger Name: tetratricopeptide repeat domain 8
• Berechnetes Molekulargewicht: 531 aa, 60 kDa
• Beobachtetes Molekulargewicht: 50-52 kDa
• GenBank-Zugangsnummer: BC026351
• Gene symbol: TTC8
• Gene ID (NCBI): 123016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.