TTDN1 Polyklonaler Antikörper

TTDN1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (3)

Kat-Nr. : 25410-1-AP

Synonyme

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

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• 

  WB analysis of NIH/3T3 using 25410-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25410-1-AP bindet in WB, IHC, ELISA TTDN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTDN1 fusion protein Ag22094
• Vollständiger Name: chromosome 7 open reading frame 11
• Berechnetes Molekulargewicht: 179 aa, 19 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC026265
• Gene symbol: TTDN1
• Gene ID (NCBI): 136647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Antibodies were used for Ttdn1 detection in RKO cells! Incubation overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

TTDN1 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:25410-1-AP)

FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect MPLKIP/TTDN1 with Western blot in HEK293T WT cell lysate, which worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT

TTDN1 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:25410-1-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Antibody detect a band in the range between 20-25 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

TTDN1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:25410-1-AP)