TTF2 Polyklonaler Antikörper

TTF2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13722-1-AP

Synonyme

HuF2, F2, EC:3.6.4.-

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  WB analysis of A2780 using 13722-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 13722-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 13722-1-AP

  IP result of anti-TTF2 (IP:13722-1-AP, 4ug; Detection:13722-1-AP 1:500) with A2780 cells lysate 840ug.

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  IHC staining of human ovary tumor using 13722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 13722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 13722-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (black).

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  IF Staining of HeLa using 13722-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: A2780-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

13722-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TTF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTF2 fusion protein Ag4671
• Vollständiger Name: transcription termination factor, RNA polymerase II
• Berechnetes Molekulargewicht: 1162 aa, 129 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC030058
• Gene symbol: TTF2
• Gene ID (NCBI): 8458
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].

Publikationen

Species	Application	Title
human	WB	Mol Cell; mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription.; Authors - Brannan Kris K
rat	WB	Sci Total Environ; PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.; Authors - Mei Ha
human	IHC	Nat Commun; Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features; Authors - Weizhu Zhao