TTL Polyklonaler Antikörper

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• KD/KO Validated

TTL Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(34)

Rezensionen (1)

Kat-Nr. : 13618-1-AP

Synonyme

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25

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• 

  WB analysis of HeLa using 13618-1-AP

  WB result of TTL antibody (13618-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells.

• 

  WB analysis using 13618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13618-1-AP (TTL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 13618-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 13618-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, mouse brain, rat brain
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 29 publications below
• IF: See 6 publications below

Produktinformation

13618-1-AP bindet in WB, IHC, IF-P, ELISA TTL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTL fusion protein Ag4526
• Vollständiger Name: tubulin tyrosine ligase
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC036819
• Gene symbol: TTL
• Gene ID (NCBI): 150465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Publikationen

Species	Application	Title
	WB	Science; Posttranslational modification of microtubules by the MATCAP detyrosinase.; Authors - Lisa Landskron
mouse	WB	Nature; MARK4 controls ischaemic heart failure through microtubule detyrosination.; Authors - Xian Yu
human	WB	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	WB	Science; Vasohibins encode tubulin detyrosinating activity.; Authors - Joppe Nieuwenhuis
human	WB	Science; Mitosis. Microtubule detyrosination guides chromosomes during mitosis.; Authors - Marin Barisic
human	WB	Nat Med; Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.; Authors - Christina Yingxian Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Multiple bands corresponding to different isoforms.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart