TTL Monoklonaler Antikörper

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TTL Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E5F8

Kat-Nr. : 66076-1-PBS

Synonyme

2E5F8, EC:6.3.2.25, Tubulin tyrosine ligase, tubulin tyrosine ligase, Tubulin--tyrosine ligase

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• 

  WB analysis using 66076-1-Ig (same clone as 66076-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66076-1-Ig (same clone as 66076-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66076-1-Ig (same clone as 66076-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66076-1-PBS bindet in WB, IHC, Indirect ELISA TTL und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TTL fusion protein Ag4708
• Vollständiger Name: tubulin tyrosine ligase
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC036819
• Gene symbol: TTL
• Gene ID (NCBI): 150465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.