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TTLL12 Polyklonaler Antikörper

TTLL12 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21447-1-PBS

Synonyme

Inactive tubulin--tyrosine ligase-like protein 12, Tubulin--tyrosine ligase-like protein 12

Filter:
  • Western Blot (WB) analysis of various lysates using TTLL12 Polyclonal antibody (21447-1-AP)

    WB analysis using 21447-1-AP (same clone as 21447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21447-1-AP (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTLL12 Polyclonal antibody (21447-1-AP)

    IHC staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21447-1-AP (TTLL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TTLL12 Polyclonal antibody (21447-1-AP)

    IF Staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTLL12 antibody (21447-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21447-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA TTLL12 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TTLL12 fusion protein Ag13804
Vollständiger Name tubulin tyrosine ligase-like family, member 12
Berechnetes Molekulargewicht 644 aa, 74 kDa
Beobachtetes Molekulargewicht 75 kDa
GenBank-ZugangsnummerBC001070
Gene symbol TTLL12
Gene ID (NCBI) 23170
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).