TTLL12 Rekombinanter Antikörper

TTLL12 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241904F4

Kat-Nr. : 84493-6-RR

Synonyme

Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12, 241904F4

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  WB analysis using 84493-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84493-6-RR (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84493-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84493-6-RR against Human TTLL12 were performed. The affinity constant is 57.3 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, DU 145-Zellen, HeLa-Zellen, K-562-Zellen, PC-3-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84493-6-RR bindet in WB, ELISA TTLL12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TTLL12 fusion protein Ag13804
• Vollständiger Name: tubulin tyrosine ligase-like family, member 12
• Berechnetes Molekulargewicht: 644 aa, 74 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC001070
• Gene symbol: TTLL12
• Gene ID (NCBI): 23170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).