TUBB3-specific/TUJ1 Rekombinanter Antikörper

TUBB3-specific/TUJ1 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250851G10

Kat-Nr. : 80128-3-PBS

Synonyme

beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB3, TUBB4

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  WB analysis using 80128-3-RR (same clone as 80128-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80128-3-RR (TUBB3-specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using TUBB3-specific antibody (80128-3-RR, Clone: 250851G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP00550-2

  The mean TUBB3 concentration was determined to be 6.4 ng/mL in SH-SY5Y cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP00550-2

  Cytometric bead array standard curve of MP00550-2, TUBB3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80128-3-PBS. Detection antibody: 80128-2-PBS. Standard: SY01676. Range: 0.039-2.5 ng/mL.

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Geprüfte Anwendungen

Produktinformation

80128-3-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA TUBB3-specific/TUJ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, beta 3
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: NM_001197181
• Gene symbol: TUBB3
• Gene ID (NCBI): 10381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.