TULP1 Polyklonaler Antikörper

TULP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18971-1-AP

Synonyme

TUBL1, Tubby-related protein 1, Tubby-like protein 1

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  WB analysis using 18971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18971-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18971-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human eye using 18971-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human eye using 18971-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, humanes Hirngewebe, Maus-Retina-Gewebe
• Erfolgreiche Detektion in IHC: humanes Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18971-1-AP bindet in WB, IHC, ELISA TULP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TULP1 fusion protein Ag5598
• Vollständiger Name: tubby like protein 1
• Berechnetes Molekulargewicht: 489 aa, 55 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC032714
• Gene symbol: TULP1
• Gene ID (NCBI): 7287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TULP1, also known as RP14, LCA15, and TUBL1, is a member of the tubby-like protein (TULP) family. TULP1 is expressed exclusively in the retina and is essential for the transport of rhodopsin from its site of synthesis in the inner segments through the connecting cilium to the outer segments(PMID: 17620573). Mutations in TULP1 are associated with Retinitis pigmentosa 14 and Leber congenital amaurosis-15(PMID: 17620573, 17962469).

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation; Authors - Mingzhu Yang