TWIST2 Polyklonaler Antikörper

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• KD/KO Validated

TWIST2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11752-1-AP

Synonyme

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 11752-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11752-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11752-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11752-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Mauslebergewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below

Produktinformation

11752-1-AP bindet in WB, IHC, ELISA TWIST2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TWIST2 fusion protein Ag2348
• Vollständiger Name: twist homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 160 aa, 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa, 60 kDa
• GenBank-Zugangsnummer: BC033168
• Gene symbol: TWIST2
• Gene ID (NCBI): 117581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Publikationen

Species	Application	Title
human	IHC	Aging (Albany NY); LINC01235-TWIST2 feedback loop facilitates epithelial-mesenchymal transition in gastric cancer by inhibiting THBS2.; Authors - Yu-En Tan
human	WB	Front Cell Dev Biol; NR2F1-AS1/miR-190a/PHLDB2 Induces the Epithelial-Mesenchymal Transformation Process in Gastric Cancer by Promoting Phosphorylation of AKT3.; Authors - Jinqi Lv
human	WB	Front Oncol; TRIM50 Suppresses Pancreatic Cancer Progression and Reverses the Epithelial-Mesenchymal Transition via Facilitating the Ubiquitous Degradation of Snail1.; Authors - Rongkun Li
mouse	WB	Neoplasia; The Heterodimeric TWIST1-E12 Complex Drives the Oncogenic Potential of TWIST1 in Human Mammary Epithelial Cells.; Authors - Laurent Jacqueroud
mouse	WB	J Cancer; Asymmetric Division Gene Neurl2 Mediates Twist2 Regulation of Self-Renewal of Mouse Lewis Lung Cancer Stem Cells.; Authors - Doudou Liu ; KD Validated
human	WB	Gene; Overexpression of NuSAP1 is predictive of an unfavourable prognosis and promotes proliferation and invasion of triple-negative breast cancer cells via the Wnt/β-catenin/EMT signalling axis.; Authors - Li Sun