TWIST2 Monoklonaler Antikörper

TWIST2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1B12D11

Kat-Nr. : 66544-1-Ig

Synonyme

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

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Galerie der Validierungsdaten

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  WB analysis using 66544-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66544-1-Ig (TWIST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human ovary tumor using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens).

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  IF Staining of HepG2 using 66544-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66544-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66544-1-Ig bindet in WB, IHC, IF, ELISA TWIST2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TWIST2 fusion protein Ag2348
• Vollständiger Name: twist homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 160 aa, 18 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC033168
• Gene symbol: TWIST2
• Gene ID (NCBI): 117581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Publikationen

Species	Application	Title
human	WB	J Transl Med; VCP interaction with HMGB1 promotes hepatocellular carcinoma progression by activating the PI3K/AKT/mTOR pathway.; Authors - Zhangya Pu
human	IHC	J Healthc Eng; Expression of Dickkopf-1 and Twist2 in Cervical Squamous Cell Carcinoma and Their Correlation with Vasculogenic Mimicry.; Authors - Beibei Wang
mouse	IF	Nat Commun; Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration; Authors - Yang Yang