Thioredoxin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Thioredoxin Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(90)

Rezensionen (1)

Kat-Nr. : 14999-1-AP

Synonyme

TXN, TRDX, THIO, Surface-associated sulphydryl protein, Hom s Trx

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  WB analysis using 14999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14999-1-AP

  WB result of Thioredoxin antibody (14999-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells.

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  IP experiment of HeLa using 14999-1-AP

  IP result of anti-Thioredoxin (IP:14999-1-AP, 4ug; Detection:14999-1-AP 1:6000) with HeLa cells lysate 1520 ug.

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  IHC staining of human liver cancer using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:900 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 14999-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Thioredoxin (14999-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 81 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• IP: See 6 publications below
• ELISA: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

14999-1-AP bindet in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA Thioredoxin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Thioredoxin fusion protein Ag6989
• Vollständiger Name: thioredoxin
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC003377
• Gene symbol: TXN
• Gene ID (NCBI): 7295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Publikationen

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	WB	Acta Pharm Sin B; Fluorine-thiol displacement probes for acetaminophen's hepatotoxicity; Authors - Benjamin L Prather
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
		Nano Converg; Detection of thioredoxin-1 using ultra-sensitive ELISA with enzyme-encapsulated human serum albumin nanoparticle.; Authors - Myeong-Jun Lee
human	WB	Cell Rep; CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons; Authors - Carissa L Sirois
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amandine (Verified Customer) (02-07-2024)

20ug of protein, denatured or not, PVDF membrane, fast transfert, primary antibody 1/5000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation 10sec

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: ImCC

Thioredoxin Antibody Western Blot validation (1/5000 dilution) in ImCC (Cat no:14999-1-AP)