TRX2 Polyklonaler Antikörper

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• KD/KO Validated

TRX2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 13089-1-AP

Synonyme

MT TRX, MTRX, Thioredoxin 2, Thioredoxin, mitochondrial, TRX2, TXN2

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Galerie der Validierungsdaten

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  WB analysis of Raji using 13089-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13089-1-AP (TRX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13089-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13089-1-AP (TRX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 13089-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13089-1-AP (TRX2 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

13089-1-AP bindet in WB, IHC, IF, ELISA TRX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRX2 fusion protein Ag3640
• Vollständiger Name: thioredoxin 2
• Berechnetes Molekulargewicht: 166 aa, 18 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC013726
• Gene symbol: TXN2
• Gene ID (NCBI): 25828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. In the retina, the expression profile and the role of Trx2 have not yet been defined (PMID: 18441302).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; G6PD protects from oxidative damage and improves healthspan in mice.; Authors - Sandrina Nóbrega-Pereira
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
human	WB	Int J Mol Sci; TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.; Authors - Tao Zhang ; KD Validated
human	WB	Free Radic Biol Med; Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21.; Authors - Kendra M Prutton
human	WB	Free Radic Biol Med; Compartmentally scavenging hepatic oxidants through AMPK/SIRT3-PGC1α axis improves mitochondrial biogenesis and glucose catabolism.; Authors - Meiling Wu