TXNDC15 Polyklonaler Antikörper

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• KD/KO Validated

TXNDC15 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32874-1-AP

Synonyme

C5orf14, Thioredoxin Domain Containing 15, Thioredoxin domain-containing protein 15, UNQ335/PRO534

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  WB analysis of HeLa using 32874-1-AP

  WB result of TXNDC15 antibody (32874-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC15 transfected HeLa cells.

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  WB analysis using 32874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32874-1-AP (TXNDC15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 32874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 32874-1-AP (TXNDC15 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32874-1-AP bindet in WB, IHC, ELISA TXNDC15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNDC15 fusion protein Ag37138
• Vollständiger Name: thioredoxin domain containing 15
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032568
• Gene symbol: TXNDC15
• Gene ID (NCBI): 79770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC15 (thioredoxin domain-containing protein 15) is a single-pass type I transmembrane protein that contains a thioredoxin domain and is primarily localized in the cytoplasm. It plays a positive regulatory role in the Hedgehog signaling pathway within cilia and actively participates in the process of ciliogenesis, exerting significant influence on the structure and function of cilia-an essential cellular organelle. Additionally, TXNDC15 has been found to form mixed disulfide complexes with endoplasmic reticulum-resident protein disulfide isomerase (PDI) family members, such as ERp57, PDI, and ERp44, suggesting its involvement in protein folding and quality control processes. Notably, mutations in the TXNDC15 gene are associated with a rare and lethal genetic disorder known as Meckel-Gruber syndrome, which is caused by defects in ciliogenesis.