TXNDC17 Polyklonaler Antikörper

TXNDC17 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20811-1-PBS

Synonyme

TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9

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  WB analysis using 20811-1-AP (same clone as 20811-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20811-1-AP (same clone as 20811-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 20811-1-AP (same clone as 20811-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20811-1-AP (same clone as 20811-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 20811-1-AP (same clone as 20811-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 20811-1-AP (same clone as 20811-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 20811-1-AP (same clone as 20811-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of human uterus using 20811-1-AP (same clone as 20811-1-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 20811-1-AP (same clone as 20811-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20811-1-AP (same clone as 20811-1-PBS)

  IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:6000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20811-1-AP (same clone as 20811-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TXNDC17 antibody (20811-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20811-1-AP (same clone as 20811-1-PBS)

  Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20811-1-AP (same clone as 20811-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green). This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20811-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA TXNDC17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNDC17 fusion protein Ag14766
• Vollständiger Name: thioredoxin domain containing 17
• Berechnetes Molekulargewicht: 123 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC006405
• Gene symbol: TXNDC17
• Gene ID (NCBI): 84817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC17 also known as TRP14, TXNL5, belongs to the thioredoxin family. TRP14 is a widely expressed, 123-amino acid protein with a WCPDC motif. TXNDC17 has a thioredoxin fold and a TRX-like active-site sequence(PMID: 14607844, 24778250).