TXNDC5 Rekombinanter Antikörper

TXNDC5 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242195G7

Kat-Nr. : 84756-5-PBS

Synonyme

Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.-

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  WB analysis using 84756-5-RR (same clone as 84756-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84756-5-RR (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (84756-5-RR, Clone: 242195G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TXNDC5 Recombinant antibody (84756-5-RR, Clone:242195G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84756-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84756-5-RR against Human TXNDC5 were performed. The affinity constant is 2.31 nM.

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Geprüfte Anwendungen

Produktinformation

84756-5-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA TXNDC5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TXNDC5 fusion protein Ag13965
• Vollständiger Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Berechnetes Molekulargewicht: 432 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC001199
• Gene symbol: TXNDC5
• Gene ID (NCBI): 81567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)