TXNDC9 Polyklonaler Antikörper

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• KD/KO Validated

TXNDC9 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25208-1-AP

Synonyme

APACD, PHLP3, Protein 1 4, TXNDC9

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 25208-1-AP

  WB result of TXNDC9 antibody (25208-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HeLa cells.

• 

  WB analysis of Raji using 25208-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25208-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25208-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 25208-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25208-1-AP (TXNDC9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25208-1-AP bindet in WB, IF, ELISA TXNDC9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNDC9 fusion protein Ag19509
• Vollständiger Name: thioredoxin domain containing 9
• Berechnetes Molekulargewicht: 226 aa, 27 kDa
• Beobachtetes Molekulargewicht: 25-27 kDa
• GenBank-Zugangsnummer: BC005968
• Gene symbol: TXNDC9
• Gene ID (NCBI): 10190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.