TXNDC9 Monoklonaler Antikörper

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• KD/KO Validated

TXNDC9 Monoklonal Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

Indirect ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

2A10G3

Kat-Nr. : 68335-1-PBS

Synonyme

Thioredoxin domain-containing protein 9, Protein 1-4, Protein 1 4, PHLP3, ATP-binding protein associated with cell differentiation

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  WB analysis using 68335-1-Ig (same clone as 68335-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68335-1-Ig (TXNDC9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68335-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68335-1-Ig (same clone as 68335-1-PBS)

  WB result of TXNDC9 antibody (68335-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HEK-293 cells. This data was developed using the same antibody clone with 68335-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68335-1-Ig (same clone as 68335-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug TXNDC9 Monoclonal antibody (68335-1-Ig, Clone:2A10G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68335-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68335-1-PBS bindet in Indirect ELISA, WB TXNDC9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TXNDC9 fusion protein Ag33259
• Vollständiger Name: thioredoxin domain containing 9
• Berechnetes Molekulargewicht: 226 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC005968
• Gene symbol: TXNDC9
• Gene ID (NCBI): 10190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.