TXNIP Polyklonaler Antikörper

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• KD/KO Validated

TXNIP Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

67

Kat-Nr. : 18243-1-AP

Synonyme

EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 18243-1-AP

  WB result of TXNIP antibody (18243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNIP transfected K-562 cells.

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  WB analysis of HL-60 using 18243-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, K-562-Zellen, MCF-7-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Ovarialkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 55 publications below
• IHC: See 18 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

18243-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TXNIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNIP fusion protein Ag13009
• Vollständiger Name: thioredoxin interacting protein
• Berechnetes Molekulargewicht: 391 aa, 44 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC093702
• Gene symbol: TXNIP
• Gene ID (NCBI): 10628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.

Publikationen

Species	Application	Title
mouse	FC	Gastroenterology; MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.; Authors - Ying Lu
human	WB	Clin Cancer Res; FBW7 (F-box and WD repeat domain-containing 7) negatively regulates glucose metabolism by targeting the c-Myc/TXNIP (thioredoxin binding protein) axis in pancreatic cancer.; Authors - Shunrong Ji
mouse	IHC	Theranostics; Epigenetic repression of miR-17 contributed to di(2-ethylhexyl) phthalate-triggered insulin resistance by targeting Keap1-Nrf2/miR-200a axis in skeletal muscle.; Authors - Jie Wei
human	WB	Redox Biol; PMID: 32330868; Authors - Yifan Jia ; KD Validated
rat	WB	Oxid Med Cell Longev; PMID: 31998437; Authors - Jing Yu
mouse	WB,IF	Oxid Med Cell Longev; PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.; Authors - Zeyu Li

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