TXNL1 Polyklonaler Antikörper

TXNL1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15289-1-AP

Synonyme

thioredoxin like 1, Thioredoxin like protein 1, TRP32, Txl, TXL 1, TXNL, TXNL1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 15289-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 15289-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 15289-1-AP

  IP Result of anti-TXNL1 (IP:15289-1-AP, 4ug; Detection:15289-1-AP 1:300) with HL-60 cells lysate 960ug.

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  IHC staining of human heart using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid cancer using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of RAW 264.7 using 15289-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 15289-1-AP (TXNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

15289-1-AP bindet in WB, IP, IHC, IF, ELISA TXNL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNL1 fusion protein Ag7481
• Vollständiger Name: thioredoxin-like 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC001156
• Gene symbol: TXNL1
• Gene ID (NCBI): 9352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNL1(Thioredoxin-like protein 1) is also named as TRP32, TXL, TXNL and belongs to the thioredoxin family. It might be involved in the cellular response to sugar starvation stress. The predicted 289-amino acid, 32.3-kD protein has 2 distinct domains: an N-terminal domain, which is 43% identical to human TXN, and a C-terminal domain, which showed no homology to other proteins in the sequence databases(PMID:9473519).TXNL1 functions as an effector of oxidants or redox sensor that couples oxidative stress to endocytosis, by converting redox changes into a specific GDI:Rab5-mediated endocytic response(PMID:17987124).

Publikationen

Species	Application	Title
human	WB,IHC	Cell Death Dis; TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer.; Authors - W Xu
human	WB	Curr Cancer Drug Targets; TXNL1 induces apoptosis in cisplatin resistant human gastric cancer cell lines.; Authors - Pan Ni
	WB	Vet Res; Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1.; Authors - Caiying Wang