TXNRD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TXNRD1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

51

Kat-Nr. : 11117-1-AP

Synonyme

GRIM 12, GRIM12, KDRF, thioredoxin reductase 1, Thioredoxin reductase TR1, TR, TR1, TRXR1, TXNR, TXNRD1

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Galerie der Validierungsdaten

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  WB analysis using 11117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11117-1-AP

  WB result of TXNRD1 antibody (11117-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNRD1 transfected HeLa cells.

• 

  WB analysis of MCF-7 using 11117-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11117-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNRD1 antibody (11117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 11117-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug TXNRD1 antibody (11117-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, MCF-7-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 45 publications below
• IHC: See 9 publications below
• IF: See 4 publications below

Produktinformation

11117-1-AP bindet in WB, IHC, IF, FC, ELISA TXNRD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNRD1 fusion protein Ag1618
• Vollständiger Name: thioredoxin reductase 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC018122
• Gene symbol: TXNRD1
• Gene ID (NCBI): 7296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Publikationen

Species	Application	Title
human	WB	Redox Biol; Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.; Authors - Angel Gallego-Selles
human,mouse	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB	Cell Death Dis; Elovanoid-N34 modulates TXNRD1 key in protection against oxidative stress-related diseases; Authors - Jorgelina M Calandria
human	WB	Cancer Res; Natural allelic variations in glutathione peroxidase-1 affect it subcellular localization and function.; Authors - Soumen Bera
rat	WB	Nucleic Acids Res; Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression.; Authors - Miniard Angela C AC
human	WB	Oxid Med Cell Longev; Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression.; Authors - Jie Qi