TXNRD1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TXNRD1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

1B10C4

Kat-Nr. : 67728-1-Ig

Synonyme

GRIM 12, GRIM12, KDRF, thioredoxin reductase 1, Thioredoxin reductase TR1, TR, TR1, TRXR1, TXNR, TXNRD1

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Galerie der Validierungsdaten

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  WB analysis using 67728-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67728-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67728-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67728-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67728-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TXNRD1 antibody (67728-1-Ig, Clone: 1B10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, 4T1-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Produktinformation

67728-1-Ig bindet in WB, IHC, IF, ELISA TXNRD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TXNRD1 fusion protein Ag30071
• Vollständiger Name: thioredoxin reductase 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-70 kDa
• GenBank-Zugangsnummer: BC018122
• Gene symbol: TXNRD1
• Gene ID (NCBI): 7296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Publikationen

Species	Application	Title
human,mouse	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
mouse	IF	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB	Front Oncol; A Robust Metabolic Enzyme-Based Prognostic Signature for Head and Neck Squamous Cell Carcinoma.; Authors - Zizhao Mai ; KD Validated
human	WB	Front Oncol; Bioinformatics-Driven Identification of p62 as A Crucial Oncogene in Liver Cancer.; Authors - Ling Wang
mouse	WB	Cell Rep; Protein S-nitrosylation regulates proteostasis and viability of hematopoietic stem cell during regeneration.; Authors - Weiwei Yi
mouse	WB	Function (Oxf); Deletion of Thioredoxin Reductase Disrupts Redox Homeostasis and Impairs β-Cell Function; Authors - Jennifer S Stancill