TYK2 Monoklonaler Antikörper

TYK2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

1E1A10

Kat-Nr. : 67411-1-Ig

Synonyme

JTK1, TYK2, tyrosine kinase 2

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  WB analysis using 67411-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 67411-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67411-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  IHC staining of human colon cancer using 67411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67411-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (67411-1-Ig, Clone: 1E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen, TF-1-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

67411-1-Ig bindet in WB, IHC, IF/ICC, ELISA TYK2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TYK2 fusion protein Ag9442
• Vollständiger Name: tyrosine kinase 2
• Berechnetes Molekulargewicht: 1187 aa, 134 kDa
• Beobachtetes Molekulargewicht: 134 kDa
• GenBank-Zugangsnummer: BC014243
• Gene symbol: TYK2
• Gene ID (NCBI): 7297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Virol; SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.; Authors - Da-Yuan Chen
human	WB	Invest New Drugs; IL-10 contributes to gemcitabine resistance in extranodal NK/T-cell lymphoma cells via ABCC4.; Authors - Jia Huo
human	WB	bioRxiv; SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.; Authors - Da-Yuan Chen
human	WB	Cancer Lett; HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma; Authors - Xueshuo Sun
mouse	WB	Antioxidants (Basel); Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I Interferon Responses; Authors - Shuni Duan
mouse	WB	Chin J Nat Med; Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses; Authors - Kexin Jia