TYMS Monoklonaler Antikörper

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• KD/KO Validated

TYMS Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1F5B7

Rezensionen (1)

Kat-Nr. : 66725-1-Ig

Synonyme

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

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Galerie der Validierungsdaten

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  WB analysis using 66725-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66725-1-Ig (TYMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66725-1-Ig

  WB result of TYMS antibody (66725-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TYMS transfected HEK-293 cells.

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  IHC staining of human colon cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66725-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TYMS antibody (66725-1-Ig, Clone: 1F5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66725-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human TYMS (66725-1-Ig, Clone:1F5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66725-1-Ig bindet in WB, IHC, IF, FC, ELISA TYMS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TYMS fusion protein Ag7027
• Vollständiger Name: thymidylate synthetase
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002567
• Gene symbol: TYMS
• Gene ID (NCBI): 7298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Publikationen

Species	Application	Title
human	WB	J Med Chem; Fuplatin: An Efficient and Low-Toxic Dual-Prodrug; Authors - Ran Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Not working

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver, mouse brain / HepG2

TYMS Antibody Western Blot validation (1:1000 dilution) in Mouse liver, mouse brain / HepG2 (Cat no:66725-1-Ig)