TYRO3 Polyklonaler Antikörper

TYRO3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28513-1-AP

Synonyme

Brt, BYK, Dtk, EC:2.7.10.1, RSE

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  WB analysis using 28513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28513-1-AP (TYRO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28513-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28513-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HCT 116 using 28513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TYRO3 antibody (28513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

28513-1-AP bindet in WB, IHC, IF/ICC, ELISA TYRO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TYRO3 fusion protein Ag29540
• Vollständiger Name: TYRO3 protein tyrosine kinase
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC049368
• Gene symbol: TYRO3
• Gene ID (NCBI): 7301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887)

Publikationen

Species	Application	Title
human	IF	Cells; TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway; Authors - Quang Duy Trinh
human	IHC	Eur Arch Otorhinolaryngol; Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps; Authors - Yandan Zhou