TYRO3 Polyklonaler Antikörper

TYRO3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28513-1-PBS

Synonyme

Brt, BYK, Dtk, EC:2.7.10.1, RSE

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  WB analysis using 28513-1-AP (same clone as 28513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28513-1-AP (TYRO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 28513-1-AP (same clone as 28513-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TYRO3 antibody (28513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28513-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TYRO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TYRO3 fusion protein Ag29540
• Vollständiger Name: TYRO3 protein tyrosine kinase
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC049368
• Gene symbol: TYRO3
• Gene ID (NCBI): 7301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887)