TYRO3 Rekombinanter Antikörper

TYRO3 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242477G1

Kat-Nr. : 84929-6-RR

Synonyme

RSE, EC:2.7.10.1, Dtk, BYK, Brt

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  WB analysis using 84929-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84929-6-RR (TYRO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84929-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84929-6-RR (TYRO3 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84929-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84929-6-RR (TYRO3 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84929-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84929-6-RR against Human TYRO3 were performed. The affinity constant is 0.133 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84929-6-RR bindet in WB, IHC, ELISA TYRO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TYRO3 fusion protein Ag29527
• Vollständiger Name: TYRO3 protein tyrosine kinase
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC049368
• Gene symbol: TYRO3
• Gene ID (NCBI): 7301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. The molecular mass of TYRO3 is 130 kDa, the apparent molecular mass is higher due to glycosylation.((PMID: 8873131, 23354874, 21539887)