TYROBP/DAP12 Polyklonaler Antikörper

TYROBP/DAP12 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 28138-1-AP

Synonyme

TYROBP, KAR-associated protein, KARAP, KAR associated protein, DNAX-activation protein 12

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  WB analysis using 28138-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 28138-1-AP (TYROBP / DAP12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28138-1-AP bindet in WB, ELISA TYROBP/DAP12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TYROBP/DAP12 fusion protein Ag27252
• Vollständiger Name: TYRO protein tyrosine kinase binding protein
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC011175
• Gene symbol: TYROBP
• Gene ID (NCBI): 7305
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TYROBP, also known as DAP12 or KARAP, is a transmembrane protein consisting of a very small extracellular region, a transmembrane domain containing an aspartic acid residue critical for association with its partner subunits, and an intracellular domain with a single immunoreceptor tyrosine-based activation motif (ITAM) (PMID: 11114420). It is expressed as a disulfide-bonded homodimer on natural killer cells, myeloid cells and a subset of T cells (PMID: 11114420). TYROBP acts as a signaling adaptor that provides signaling function via the Syk and ZAP-70 tyrosine kinase activation pathways (PMID: 15895090). The gene of TYROBP is located on the long arm of chromosome 19 at position 13.1. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene.

Publikationen

Species	Application	Title
mouse	WB	Phytomedicine; Berberine modulates microglial polarization by activating TYROBP in Alzheimer's disease; Authors - Yu Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katia (Verified Customer) (09-09-2025)

The antibody performed well on mouse brain homogenate. However, it did not yield a signal in primary cells homogenate, possibly due to lower protein loading. Notably, a different DAP12 antibody worked under the same conditions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain - mouse primary cells