Tenascin-R Polyklonaler Antikörper

Tenascin-R Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19730-1-PBS

Synonyme

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  WB analysis of human brain using 19730-1-AP (same clone as 19730-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19730-1-AP (Tenascin-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19730-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 19730-1-AP (same clone as 19730-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19730-1-AP (Tenascin-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 19730-1-AP (same clone as 19730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19730-1-AP (Tenascin-R antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 19730-1-AP (same clone as 19730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19730-1-AP (Tenascin-R antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19730-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19730-1-PBS bindet in WB, IHC, Indirect ELISA Tenascin-R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tenascin R (restrictin, janusin)
• Berechnetes Molekulargewicht: 150 kDa
• Beobachtetes Molekulargewicht: 180 kDa, 160 kDa
• GenBank-Zugangsnummer: NM_003285
• Gene symbol: TNR
• Gene ID (NCBI): 7143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNR, also named as Restrictin and Janusin, belongs to the tenascin family. Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides, TNR inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides, TNR results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1, TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B, TNR may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. The antibody is specific to TNR.