Tenascin-X Polyklonaler Antikörper

Tenascin-X Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13595-1-PBS

Synonyme

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  WB analysis of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13595-1-AP (same clone as 13595-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 13595-1-AP (same clone as 13595-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

  IP result of anti-Tenascin-X (IP:13595-1-AP, 4ug; Detection:13595-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13595-1-PBS bindet in WB, IHC, IP, Indirect ELISA Tenascin-X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Tenascin-X fusion protein Ag4501
• Vollständiger Name: tenascin XB
• Berechnetes Molekulargewicht: 4289 aa, 464 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 140 kDa
• GenBank-Zugangsnummer: BC033740
• Gene symbol: TNXB
• Gene ID (NCBI): 7148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tenascin-X (TNXB) is an extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) (PMID: 23768946). It interacts with many other ECM proteins and it accelerates collagen fibrillogenesis in vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band of about 75 kDa, which probably represents a proteolytic cleaved form of human TNXB (PMID: 17263730). But the serum form of tenascin-X is a 140-kD protein probably resulting from proteolytic cleavage or alternative splicing of tenascin-X(PMID: 16567571, 11642233).