CD141/Thrombomodulin Rekombinanter Antikörper

CD141/Thrombomodulin Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240365D1

Kat-Nr. : 83416-4-PBS

Synonyme

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

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  WB analysis using 83416-4-RR (same clone as 83416-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83416-4-RR (Thrombomodulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IHC staining of human lung squamous cell carcinoma using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83416-4-RR (same clone as 83416-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thrombomodulin antibody (83416-4-RR, Clone: 240365D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83416-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83416-4-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 1.15 nM.

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Geprüfte Anwendungen

Produktinformation

83416-4-PBS bindet in WB, IHC, IF/ICC, ELISA CD141/Thrombomodulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: thrombomodulin
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: NM_000361.3
• Gene symbol: THBD
• Gene ID (NCBI): 7056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).