CD141/Thrombomodulin Rekombinanter Antikörper

CD141/Thrombomodulin Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241730D4

Kat-Nr. : 84412-3-PBS

Synonyme

241730D4, CD141, Fetomodulin, Thbd, Thrombomodulin

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  WB analysis of mouse placenta using 84412-3-RR (same clone as 84412-3-PBS)

  mouse placenta tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 84412-3-RR (same clone as 84412-3-PBS)

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01300-2

  Sandwich ELISA standard curve of MP01300-2, Mouse CD141/Thrombomodulin Recombinant Matched Antibody Pair - PBS only. 84412-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1139. 84412-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Sample test of MP01300-2

  Serum of six mice was measured. The mouse CD141/Thrombomodulin concentration of detected samples was determined to be 28.01 ng/mL with a range of 21.90 - 32.07 ng/mL.

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  Sample test of MP01300-2

  Mouse lung, cut into 1-2 mm pieces, wa scultured for 4 daysin 25-30 mL of RPMI supplemented with 10% fetal bovine serum. The cell culture supernate was removed, assayed for CD141/Thrombomodulin, and measured 34.22 ng/mL.

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  IHC staining of mouse stomach using 84412-3-RR (same clone as 84412-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 84412-3-RR (same clone as 84412-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84412-3-RR (same clone as 84412-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84412-3-RR (same clone as 84412-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

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  IF Staining of bEnd.3 using 84412-3-RR (same clone as 84412-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD141/Thrombomodulin antibody (84412-3-RR, Clone: 241730D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01300-2

  Cytometric bead array standard curve of MP01300-2, MOUSE CD141/Thrombomodulin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84412-3-PBS. Detection antibody: 84412-1-PBS. Standard: Eg1139. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84412-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84412-3-RR against Mouse CD141/Thrombomodulin were performed. The affinity constant is 3.11 nM.

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Geprüfte Anwendungen

Produktinformation

84412-3-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD141/Thrombomodulin und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD141/Thrombomodulin fusion protein Eg1139
• Vollständiger Name: thrombomodulin
• Berechnetes Molekulargewicht: 62kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: NM_009378.3
• Gene symbol: Thbd
• Gene ID (NCBI): 21824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).