Thrombopoietin Rekombinanter Antikörper

Thrombopoietin Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250023B3

Kat-Nr. : 83549-7-RR

Synonyme

Thpo, Mpllg, TPO

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  WB analysis using 83549-7-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83549-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 83549-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 83549-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, HuH-7-Zellen, L02-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83549-7-RR bindet in WB, IHC, ELISA Thrombopoietin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Thrombopoietin fusion protein Eg0678
• Vollständiger Name: thrombopoietin
• Berechnetes Molekulargewicht: 38KD
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_001173505.1
• Gene symbol: Thrombopoietin
• Gene ID (NCBI): 21832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).