Thrombospondin 1 Monoklonaler Antikörper

Thrombospondin 1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2A3E1

Kat-Nr. : 67241-1-Ig

Synonyme

THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1

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Galerie der Validierungsdaten

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  WB analysis using 67241-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67241-1-Ig (THBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 67241-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67241-1-Ig (THBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67241-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67241-1-Ig (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, Rattenmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67241-1-Ig bindet in WB, ELISA Thrombospondin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Thrombospondin 1 fusion protein Ag29129
• Vollständiger Name: thrombospondin 1
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_003246
• Gene symbol: THBS1
• Gene ID (NCBI): 7057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Publikationen

Species	Application	Title
rat	WB	Diabetes Metab Syndr Obes; Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats; Authors - Yu Ni