Tie-2/CD202b Polyklonaler Antikörper

Tie-2/CD202b Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19157-1-PBS

Synonyme

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

---

• 

  WB analysis of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie-2/CD202b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19157-1-AP (same clone as 19157-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

  IP result of anti-Tie2 (IP:19157-1-AP, 4ug; Detection:19157-1-AP 1:500) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 19157-1-AP (same clone as 19157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

19157-1-PBS bindet in WB, IHC, IP, Indirect ELISA Tie-2/CD202b und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Tie-2/CD202b fusion protein Ag13523
• Vollständiger Name: TEK tyrosine kinase, endothelial
• Berechnetes Molekulargewicht: 1124 aa, 126 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC035514
• Gene symbol: Tie2
• Gene ID (NCBI): 7010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.