TIM23 Monoklonaler Antikörper

TIM23 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

3H10F5

Kat-Nr. : 67535-1-Ig

Synonyme

67535, TIMM23, 3H10F5, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23

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  WB analysis using 67535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67535-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

67535-1-Ig bindet in WB, IHC, IF/ICC, ELISA TIM23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TIM23 fusion protein Ag27069
• Vollständiger Name: translocase of inner mitochondrial membrane 23
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC062707
• Gene symbol: TIMM23
• Gene ID (NCBI): 100287932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Publikationen

Species	Application	Title
human	WB	Autophagy; The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy; Authors - Bo Zhang
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	WB,IF	Autophagy; Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.; Authors - Yinjuan Song
mouse	WB	Mol Med; 4-Octyl itaconate attenuates LPS-induced acute kidney injury by activating Nrf2 and inhibiting STAT3 signaling; Authors - Lujun Xu
human	WB	Zool Res; Choline dehydrogenase interacts with SQSTM1 to activate mitophagy and promote coelomocyte survival in Apostichopus japonicus following Vibrio splendidus infection; Authors - Lian-Lian Sun
mouse,human	WB	Front Pharmacol; Low-dose of caffeine alleviates high altitude pulmonary edema via regulating mitochondrial quality control process in AT1 cells; Authors - Liuyang Tian