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TIM23 Monoklonaler Antikörper

TIM23 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H10F5

Kat-Nr. : 67535-1-PBS

Synonyme

67535, TIMM23, 3H10F5, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23

Filter:
  • Western Blot (WB) analysis of various lysates using TIM23 Monoclonal antibody (67535-1-Ig)

    WB analysis using 67535-1-Ig (same clone as 67535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (TIM23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIM23 Monoclonal antibody (67535-1-Ig)

    IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (TIM23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TIM23 Monoclonal antibody (67535-1-Ig)

    IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (TIM23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TIM23 Monoclonal antibody (67535-1-Ig)

    IF Staining of HepG2 using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67535-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TIM23 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TIM23 fusion protein Ag27069
Vollständiger Name translocase of inner mitochondrial membrane 23
Berechnetes Molekulargewicht 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC062707
Gene symbol TIMM23
Gene ID (NCBI) 100287932
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.