Timp-3 Polyklonaler Antikörper

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• KD/KO Validated

Timp-3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 10858-1-AP

Synonyme

HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, Protein MIG 5, SFD, Timp 3, TIMP3, Timp-3

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Galerie der Validierungsdaten

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  WB analysis using 10858-1-AP

  Human placenta tissue lysates were subjected to SDS PAGE followed by western blot with 10858-1-AP (Timp-3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10858-1-AP (Timp-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10858-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10858-1-AP (Timp-3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10858-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10858-1-AP (Timp-3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 8 publications below

Produktinformation

10858-1-AP bindet in WB, IHC, ELISA Timp-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Timp-3 fusion protein Ag1253
• Vollständiger Name: TIMP metallopeptidase inhibitor 3
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24-33 kDa
• GenBank-Zugangsnummer: BC014277
• Gene symbol: TIMP3
• Gene ID (NCBI): 7078
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Theranostics; miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.; Authors - Jing Shi
human	IHC	Oxid Med Cell Longev; Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A.; Authors - Li Li
human		Int Immunopharmacol; Sublytic C5b-9 induces TIMP3 expression by glomerular mesangial cells via TRAF6-dependent KLF5 K63-linked ubiquitination in rat Thy-1 nephritis; Authors - Shuai Ying
	IHC	Sci Rep; AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice.; Authors - Sheng Zhou
	IHC	Cell Prolif; Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.; Authors - Yunjiao Wang
		Biomolecules; Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse Model.; Authors - Sophie Schmidt

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