Tissue Factor/CD142 Rekombinanter Antikörper

Tissue Factor/CD142 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240875H3

Kat-Nr. : 83887-4-RR

Synonyme

CD142, Coagulation factor III, F3, Tissue factor, 240875H3

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  WB analysis using 83887-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 83887-4-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83887-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhautgewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83887-4-RR bindet in WB, ELISA Tissue Factor/CD142 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Tissue Factor/CD142 fusion protein Eg1102
• Vollständiger Name: coagulation factor III
• Berechnetes Molekulargewicht: 33kDa
• Beobachtetes Molekulargewicht: 48-53 kDa
• Gene symbol: Tissue Factor
• Gene ID (NCBI): 14066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.