Titin Polyklonaler Antikörper

Titin Polyklonal Antikörper für IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27867-1-PBS

Synonyme

TTN

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  IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 27867-1-AP (same clone as 27867-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Titin antibody (27867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27867-1-PBS bindet in IHC, IF/ICC, IF-P, Indirect ELISA Titin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Titin fusion protein Ag27496
• Vollständiger Name: titin
• Berechnetes Molekulargewicht: 3816 kDa
• GenBank-Zugangsnummer: NM_001256850
• Gene symbol: Titin
• Gene ID (NCBI): 7273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Titin, or connectin, is a giant muscle protein expressed in the cardiac and skeletal muscles that spans half of the sarcomere from Z line to M line. It plays a key role in muscle contraction and relaxation, maintaining the structural integrity of the myotome(PMID: 11846417). Mutations in Titin are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma(PMID: 15802564).