TLR4 Polyklonaler Antikörper

TLR4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 30400-1-AP

Synonyme

CD284, toll like receptor 4

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  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, Mauslebergewebe, Mausmilzgewebe, Rattenlebergewebe, Rattenmilzgewebe, RAW 264.7-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

30400-1-AP bindet in WB, IF, ELISA TLR4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLR4 fusion protein Ag32998
• Vollständiger Name: toll-like receptor 4
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: NM_021297
• Gene symbol: Tlr4
• Gene ID (NCBI): 21898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR4, also known as CD284, belongs to the Toll-like receptor family. It is a type I transmembrane protein consisting of an extracellular leucine-rich repeat region, a helix transmembrane domain, and an intracellular Toll-IL-1 receptor domain. TLR4 functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. It interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response.

Publikationen

Species	Application	Title
mouse	WB,IF	Free Radic Biol Med; Short-term exposure to low doses of aflatoxin B1 aggravates nonalcoholic steatohepatitis by TLR4-mediated necroptosis; Authors - Junya Zeng
mouse	WB	Brain Behav Immun Health; Interleukin-18 interacts with NKCC1 to mediate brain injury after intracerebral hemorrhage; Authors - Beibei Xu
rat,mouse	WB	Heliyon; Asprosin promotes vascular inflammation via TLR4-NFκB-mediated NLRP3 inflammasome activation in hypertension; Authors - Rui Ge
mouse	WB	ACS Appl Mater Interfaces; Clinical Grade Fibroin Sutures with Bioactive Gold Clusters Enhance Surgical Wound Healing via Inflammation Modulation; Authors - Fangzhen Tian
mouse	WB	Int J Immunopathol Pharmacol; Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells; Authors - Yujie Wang ; KD Validated
mouse	WB	Adv Healthc Mater; Atomic Artificial Enzyme for Acute and Chronic Pneumonia; Authors - Wei Liu