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TNF-alpha Rekombinanter Antikörper

TNF-alpha Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240702B7

Kat-Nr. : 80258-6-PBS

Synonyme

Tnf, Tnfa, TNF alpha,TNF-a,TNF-alpha,TNFα, TNF alpha, TNF a

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using TNF-alpha Recombinant antibody (80258-6-RR)

    WB analysis of RAW 264.7 using 80258-6-RR (same clone as 80258-6-PBS)

    PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80258-6-RR (Tnfa antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80258-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using TNF-alpha Recombinant antibody (80258-6-RR)

    IF Staining of RAW 264.7 using 80258-6-RR (same clone as 80258-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Tnfa antibody (80258-6-RR, Clone: 240702B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80258-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80258-6-RR

    Affinity and Kinetic Characterization of 80258-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80258-6-RR against Mouse Tnfa were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

80258-6-PBS bindet in WB, IF/ICC, ELISA TNF-alpha und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name tumor necrosis factor
Beobachtetes Molekulargewicht25-26 kDa
GenBank-ZugangsnummerNM-013693
Gene symbol TNF-alpha
Gene ID (NCBI) 21926
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.