Transferrin Receptor 2/TFR2 Polyklonaler Antikörper

Transferrin Receptor 2/TFR2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32381-1-PBS

Synonyme

TFR2, Transferrin receptor protein 2, transferrin receptor 2, TFRC2, HFE3

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  WB analysis using 32381-1-AP (same clone as 32381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32381-1-AP (TFR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32381-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 32381-1-AP (same clone as 32381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32381-1-AP (Transferrin Receptor 2/TFR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32381-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

32381-1-PBS bindet in WB, IHC, Indirect ELISA Transferrin Receptor 2/TFR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Transferrin Receptor 2/TFR2 fusion protein Ag37959
• Vollständiger Name: transferrin receptor 2
• Berechnetes Molekulargewicht: 801 aa, 89 kDa
• Beobachtetes Molekulargewicht: 88-97 kDa
• GenBank-Zugangsnummer: BC142630
• Gene symbol: TFR2
• Gene ID (NCBI): 7036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transferrin Receptor 2 (TFR2) is a type II transmembrane glycoprotein that plays a crucial role in iron homeostasis. It is highly homologous to Transferrin Receptor 1 (TFR1) and is primarily expressed in the liver and erythroid precursor cells. TFR2 is involved in the regulation of hepcidin, a hormone that controls iron absorption and distribution in the body. It helps mediate the cellular uptake of iron-bound transferrin, although its affinity for iron is lower than that of TFR1 (PMID: 24658816). It is more involved in sensing iron levels and regulating hepcidin expression rather than direct iron uptake. TFR2 is predominantly expressed in hepatocytes (liver cells) and erythroid precursors (cells that give rise to red blood cells). It is also found in the enterocytes of the small intestine (PMID: 29969719). In the liver, TFR2 acts as a sensor for circulating iron-bound transferrin and helps regulate hepcidin production. Hepcidin, in turn, controls iron absorption from the diet and iron release from macrophages. The molecular weight of TFR2 is 88 kDa, its observed molecular weight is 88-97 kDa.