Transferrin/TF Polyklonaler Antikörper

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• KD/KO Validated

Transferrin/TF Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17435-1-PBS

Synonyme

TF, transferrin, Beta 1 metal binding globulin, Beta-1 metal-binding globulin, PRO1557

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  WB analysis using 17435-1-AP (same clone as 17435-1-PBS)

  human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17435-1-AP (same clone as 17435-1-PBS)

  WB result of Transferrin/TF antibody (17435-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Transferrin/TF transfected HepG2 cells. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 17435-1-AP (same clone as 17435-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 17435-1-AP (same clone as 17435-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 17435-1-AP (same clone as 17435-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 17435-1-AP (same clone as 17435-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 17435-1-AP (same clone as 17435-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17435-1-AP (Transferrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17435-1-AP (same clone as 17435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 17435-1-AP (same clone as 17435-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Transferrin/TF antibody (17435-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 17435-1-AP (same clone as 17435-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17435-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Transferrin/TF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Transferrin/TF fusion protein Ag11504
• Vollständiger Name: transferrin
• Berechnetes Molekulargewicht: 698 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC059367
• Gene symbol: TF
• Gene ID (NCBI): 7018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273