Transgelin 2 Monoklonaler Antikörper

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• KD/KO Validated

Transgelin 2 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E5G1

Kat-Nr. : 60044-1-PBS

Synonyme

TAGLN2, 2E5G1, CDABP0035, Epididymis tissue protein Li 7e, SM22-alpha homolog

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  WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60044-1-Ig (same clone as 60044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60044-1-Ig (same clone as 60044-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

  IP result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60044-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Transgelin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Transgelin 2 fusion protein Ag0337
• Vollständiger Name: transgelin 2
• Berechnetes Molekulargewicht: 199 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC002616
• Gene symbol: Transgelin-2/TAGLN2
• Gene ID (NCBI): 8407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.