Transketolase Monoklonaler Antikörper

Transketolase Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E2F3

Kat-Nr. : 66016-1-PBS

Synonyme

TKT, TK, EC:2.2.1.1, 3E2F3

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  WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66016-1-Ig (same clone as 66016-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66016-1-Ig (same clone as 66016-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transketolase antibody (66016-1-Ig, Clone: 3E2F3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66016-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Transketolase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Transketolase fusion protein Ag16779
• Vollständiger Name: transketolase
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC009970
• Gene symbol: Transketolase
• Gene ID (NCBI): 7086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.