Prealbumin/transthyretin Rekombinanter Antikörper

Prealbumin/transthyretin Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250820B6

Kat-Nr. : 86273-1-PBS

Synonyme

Prealbumin, transthyretin, TTR, ATTR, HsT2651

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  WB analysis using 86273-1-RR (same clone as 86273-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86273-1-RR (Transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86273-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02304-2

  Sandwich ELISA standard curve of MP02304-2, Human Prealbumin/transthyretin Recombinant Matched Antibody Pair - PBS only. 86273-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0931. 86273-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

• 

  Sample test of MP02304-2

  Serum of six individual healthy human donors was measured. The Prealbumin/transthyretin concentration of detected samples was determined to be 531.06 μg/mL with a range of 290.57-729.62 μg/mL.

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  Sample test of MP02304-1

  The mean Prealbumin/TTR concentration was determined to be 172.8 ug/mL in human serum.

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  Sample test of MP02304-2

  Saliva of six individual healthy human donors was measured. The Prealbumin/transthyretin concentration of detected samples was determined to be 116.83 ng/mL with a range of 38.22-308.96 ng/mL.

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  Sample test of MP02304-2

  Human milk of six individual healthy human donors was measured. The Prealbumin/transthyretin concentration of detected samples was determined to be 1,786.26 ng/mL with a range of 1,451.02-2,605.42 ng/mL.

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  Sample test of MP02304-2

  Urine of six individual healthy human donors was measured. The Prealbumin/transthyretin concentration of detected samples was determined to be 9.27 ng/mL with a range of 0.09-45.04 ng/mL.

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  Sample test of MP02304-2

  Human cerebrospinal fluid (CSF) samples were evaluated for the presence of human Prealbumin/transthyretin in this assay, and measured 18.57 μg/mL.

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  Sample test of MP02304-2

  HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum. An aliquot of the cell culture supernate was removed, assayed for human Prealbumin/transthyretin, and measured 2.14 ng/mL.

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  Cytometric bead array standard curve of MP02304-1

  Cytometric bead array standard curve of MP02304-1, Prealbumin/TTR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86273-2-PBS. Detection antibody: 86273-1-PBS. Standard: Eg0931. Range: 1.56-50 ng/mL.

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  Affinity and Kinetic Characterization of 86273-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86273-1-RR against Human Prealbumin/transthyretin were performed. The affinity constant is 0.268 nM.

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Geprüfte Anwendungen

Produktinformation

86273-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Prealbumin/transthyretin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Prealbumin/transthyretin fusion protein Eg0931
• Vollständiger Name: transthyretin
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: NM_000371.4
• Gene symbol: TTR
• Gene ID (NCBI): 7276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)