TrkB Polyklonaler Antikörper

TrkB Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29961-1-AP

Synonyme

NTRK2, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB, NTRK 2

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  WB analysis using 29961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (NTRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (TrkB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 29961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TrkB antibody (29961-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

29961-1-AP bindet in WB, IHC, IF-P, ELISA TrkB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TrkB fusion protein Ag32196
• Vollständiger Name: neurotrophic tyrosine kinase, receptor, type 2
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa, 140-145 kDa
• GenBank-Zugangsnummer: BC031835
• Gene symbol: TrkB
• Gene ID (NCBI): 4915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 238-430 aa of human TrkB recognizes full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Publikationen

Species	Application	Title
rat	WB	J Cell Biochem; Circ-ECH1 May Compete With miR-708-5p to Regulate Ntrk2 in Bronchopulmonary Dysplasia; Authors - Hanrong Cheng
mouse	WB	Mol Neurobiol; Alpha‑Asarone Ameliorates Neuronal Injury After Ischemic Stroke and Hemorrhagic Transformation by Attenuating Blood-Brain Barrier Destruction, Promoting Neurogenesis, and Inhibiting Neuroinflammation; Authors - Lijun Luo